Plasmid

Part:BBa_K5302018:Design

Designed by: Xinyuan Shi   Group: iGEM24_USTC   (2024-10-01)


pBBR-OmpA-8IJZ


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1920
    Illegal XbaI site found at 3081
    Illegal PstI site found at 2188
    Illegal PstI site found at 3093
    Illegal PstI site found at 4254
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1920
    Illegal PstI site found at 2188
    Illegal PstI site found at 3093
    Illegal PstI site found at 4254
    Illegal NotI site found at 5211
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1920
    Illegal BglII site found at 4467
    Illegal BamHI site found at 3075
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1920
    Illegal XbaI site found at 3081
    Illegal PstI site found at 2188
    Illegal PstI site found at 3093
    Illegal PstI site found at 4254
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1920
    Illegal XbaI site found at 3081
    Illegal PstI site found at 2188
    Illegal PstI site found at 3093
    Illegal PstI site found at 4254
    Illegal NgoMIV site found at 1262
    Illegal NgoMIV site found at 3520
    Illegal NgoMIV site found at 3803
    Illegal AgeI site found at 1422
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4891
    Illegal SapI site found at 3743
    Illegal SapI site found at 3953


Design Notes

Please be advised that all listed genes have undergone codon optimization for our chassis strain, Escherichia coli Nissle 1917 (EcN). For those considering alternative chassis organisms, it is recommended to reassess the necessity of codon optimization.


Source

OmpA (Outer membrane protein A) from Escherichia coli str. K-12 substr. MG1655. 8IJZ was changed from 8IIU through several point mutations, source organism of 8IIU: Homo sapiens

References